In this large room are on displey some of the most important artworks of the ferrarese painter Lorenzo Costa, coming from Bolognese churches: the Madonna enthroned with Child and Saints Petronius and Tecla, aka Saint Tecla Altarpiece, in the namesake church, one of his first experience with classicism, and the St. Petronius with the saints Francis and Dominic, from the Annunziata church.

Even the Assumpted Madonna from Monteveglio Abbey, a temper on canvas from the 1590's.

In this room can be found even a painting of the Ferrarese Sebastiano Manieri depicting Saint Sebastian, the Baruffino Portrait by Giovan Francesco Maineri and a painting by some member of the Lendinara entourage.