This room is dedicated mainly to the Madonna with Child and Saints Margaret, Hyeronymus and Petronius (1529) by the Parmigianino, an artworks where the formal elegance and the ambivalent expression of mannerism are wonderfully combined.

Girolamo da Cotignola, remembered with his Bolognese fellows in the church of San Michele in Bosco and in several locations open in Bologna at the begnning of the XVIth century, is here represented with his Madonna with Child, St. John the Baptist and Bermard, where it can be found elements by Raphael, and of Venice and Bologna school, and the large altarpiece with the Marriage of the Virgin, with its altar-step with the St. Joseph's Dream, Jesus' Birth and Flee into Egypt, Sogno di San Giuseppe. Natività di Cristo. Fuga in Egitto, in which raphael style, central-Italy memories and Bolognese culture are united in one of the most complex results of his art.